Detailed view for Tb927.8.5990

Basic information

TDR Targets ID: 13581
Trypanosoma brucei, NLE (NUC135) domain/WD domain, G-beta repeat, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.123 | Length (AA): 524 | MW (Da): 58287 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00400   WD domain, G-beta repeat
PF08154   NLE (NUC135) domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127223)

Species Accession Gene Product
Arabidopsis thaliana AT5G52820   Notchless protein homolog
Babesia bovis BBOV_II003080   WD-repeat protein, putative
Brugia malayi Bm1_16265   WD-repeat protein HUSSY-07
Candida albicans CaO19.11260   three WD-40 domains
Candida albicans CaO19.11259   five WD-40 domains
Candida albicans CaO19.3778   five WD-40 domains
Candida albicans CaO19.3779   three WD-40 domains
Caenorhabditis elegans CELE_W07E6.2   Protein W07E6.2
Cryptosporidium hominis Chro.20040   notchless
Cryptosporidium parvum cgd2_330   notchless
Dictyostelium discoideum DDB_G0295761   NLE domain-containing protein
Drosophila melanogaster Dmel_CG2863   Notchless
Echinococcus granulosus EgrG_001015100   notchless 1
Entamoeba histolytica EHI_130870   WD domain containing protein
Echinococcus multilocularis EmuJ_001015100   notchless 1
Giardia lamblia GL50803_13667   Notchless
Homo sapiens ENSG00000073536   notchless homolog 1 (Drosophila)
Leishmania braziliensis LbrM.24.2340   notchless homolog, putative
Leishmania donovani LdBPK_242350.1   notchless homolog, putative
Leishmania infantum LinJ.24.2350   notchless homolog, putative
Leishmania major LmjF.24.2260   notchless homolog, putative
Leishmania mexicana LmxM.24.2260   notchless homolog, putative
Loa Loa (eye worm) LOAG_02482   WD-repeat protein HUSSY-07
Mus musculus ENSMUSG00000020692   notchless homolog 1 (Drosophila)
Neospora caninum NCLIV_052610   hypothetical protein, conserved
Oryza sativa 4347950   Os10g0104500
Oryza sativa 4350837   Os11g0594200
Onchocerca volvulus OVOC13494  
Plasmodium berghei PBANKA_0903000   ribosome assembly protein 4, putative
Plasmodium falciparum PF3D7_1146000   ribosome assembly protein 4, putative
Plasmodium knowlesi PKNH_0943900   ribosome assembly protein 4, putative
Plasmodium vivax PVX_092900   WD domain, G-beta repeat domain containing protein
Plasmodium yoelii PY02598   notchless-related
Saccharomyces cerevisiae YCR072C   Rsa4p
Schistosoma japonicum Sjp_0013780   Notchless protein homolog 1, putative
Schistosoma mansoni Smp_000600   notchless homolog 1
Schmidtea mediterranea mk4.019277.01   Protein Notchless
Schmidtea mediterranea mk4.004217.02   Notchless-1
Trypanosoma brucei gambiense Tbg972.8.5990   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.5990   NLE (NUC135) domain/WD domain, G-beta repeat, putative
Trypanosoma congolense TcIL3000_8_5760   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511897.28   NLE (NUC135) domain/WD domain, G-beta repeat, putative
Trypanosoma cruzi TcCLB.511075.20   notchless homolog, putative
Toxoplasma gondii TGME49_215740   notchless, putative
Theileria parva TP04_0285   hypothetical protein
Trichomonas vaginalis TVAG_356470   WD repeat domain, putative

Essentiality

Tb927.8.5990 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5990 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5990 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.5990 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.5990 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_W07E6.2 Caenorhabditis elegans larval arrest wormbase
CELE_W07E6.2 Caenorhabditis elegans slow growth wormbase
CELE_W07E6.2 Caenorhabditis elegans sterile wormbase
YCR072C Saccharomyces cerevisiae inviable yeastgenome
TGME49_215740 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.8.5990 (Trypanosoma brucei), NLE (NUC135) domain/WD domain, G-beta repeat, putative
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